The principal research interest of the lab is to comprehensively understand the biology of specific cancer types that are common in India and/or are distinct from their western counterpart(s). To this end, we use genomic, molecular genetic, molecular pathology and cell biology tools to identify and characterize recurrent genetic alterations in tumor samples.
Positions - We are currently seeking potential PhD students and Postdoc candidates to work on two major projects. One involves characterisation of PARD6G, a poorly studied member of the epithelial cell apico-basal polarity complex. The project shall include application of several cell biology based experiments in cancer cell lines, evaluation of tumorigenicity in nude mice model systems as well as CRISPR-Cas9 based knockout in Zebra Fish model systems (collaboration with the Sonawane lab at TIFR, Mumbai). The other proposes to increase our understanding of differential roles of ARID1A & ARID1B, the two alternative DNA binding subunits of the SWI/SNF chromatin remodelling complex. The work comprises proteome based screen for interactive partners, ChIP and RNA Seq and further characterisation.
Details of current projects are given below:
Characterisation of gain of function activity of non-hotspot p53 mutations identified from Indian squamous cell carcinoma patients using the CRISPR/Cas9 system.
Identification and characterization of pancreatic cancer genes located within novel localized copy number alterations.
a. Studies on role of PARD6G, a putative novel tumor suppressor, in apico-basal polarity
b. Characterization of divergent functions of ARID1A and ARID1B: the two alternative DNA binding constituents of the human SWI/SNF chromatin remodeling complex
Molecular Characterization of early-onset rectal cancer.
Molecular analysis of genetic disorders.