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"LEARN FROM THE MASTERS"
CDFD LECTURE SERIES
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"The quiescent genome: Balancing options, anticipating change"
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by
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Dr Jyotsna Dhawan
CSIR Center for Cellular & Molecular Biology, Hyderabad, India & Institute for Stem Cell Biology &
Regenerative Medicine, Bangalore
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Wednesday, 26 April, 2017
10:30 AM
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at CDFD Lecture Hall, 2nd Floor,
Lab Building.
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Abstract:
Adult stem cells persist within tissues in a quiescent state (G0), and when activated by damage, contribute to regeneration of muscle fibers. Growing evidence supports the view that G0 is a balanced or poised state where the cell cycle as well as tissue-specific programs are held in check by active mechanisms. We employ a comparative approach to understanding quiescence in mammalian skeletal muscle stem cells and culture models, contrasting G0 to terminal differentiation, another mitotically inactive state, where distinct molecular mechanisms drive a myogenic program to create multinucleate contractile myotubes. Our studies indicate that G0 myoblasts induce specific regulatory networks that permit the return of the quiescent cell to active division. These networks operate at the multiple levels from chromatin configuration all the way to translation and signaling, to ensure that repressed cell cycle genes are poised for activation. In my talk I will focus on our recent genome-wide analysis of RNA polymerase II location and activity that supports the emerging picture that polymerase pausing also contributes to maintenance of the poised state. I will discuss the hypothesis that these findings at epigenetic and transcriptional levels represent anticipatory regulatory mechanisms that allow re-entry from quiescence, and augment self-renewal capacity.
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